Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Characterization of the Salmonella typhi outer membrane protein C

(2013) Characterization of the Salmonella typhi outer membrane protein C. Korean Journal of Microbiology and Biotechnology. pp. 128-134. ISSN 1598642X (ISSN)

Full text not available from this repository.

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Salmonella enterica serovar typhi, a Gram-negative food-borne pathogen, causes typhoid fever in humans. OmpC is an outer membrane porin of S. typhi expressed throughout the infection period. OmpC is potentially an attractive antigen for multivalent vaccines and diagnostic kit designs. In this study we combined in silico, in vitro and in vivo approaches to analyze various aspects of OmpC's antigenic properties. The conserved region, in addition to secondary and tertiary structures, and linear B cell epitopes, were predicted. A number of results obtained from in silico analyses were validated by experimental studies. OmpC was amplified, cloned and then expressed, with the recombinant protein then being purified. BALB/c mice were immunized by purified denatured OmpC. The titer of antibody was raised. Results of challenges with the pathogen revealed that the immunity is non-protective. Most of the theoretical and experimental results were in consensus. Introduced linear B cell epitopes can be employed for the design of diagnostic kits based on antigen-antibody interactions.

Item Type: Article
Keywords: Diagnosis OmpC Salmonella typhi in silico Typhoid Vaccine immunoglobulin G outer membrane protein C porin antibody titer antigenicity article bacterial load computer model controlled study immunization immunogenicity immunoreactivity in vitro study in vivo study male molecular cloning mouse nonhuman protein analysis protein expression protein function protein purification Salmonella typhi Mus Negibacteria Salmonella enterica subsp. enterica serovar Typhi
Divisions:
Page Range: pp. 128-134
Journal or Publication Title: Korean Journal of Microbiology and Biotechnology
Journal Index: Scopus
Volume: 41
Number: 1
Identification Number: https://doi.org/10.4014/kjmb.1207.07009
ISSN: 1598642X (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/1038

Actions (login required)

View Item View Item