Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Anti-leishmanial activities of selenium nanoparticles and selenium dioxide on Leishmania infantum

(2014) Anti-leishmanial activities of selenium nanoparticles and selenium dioxide on Leishmania infantum. Comparative Clinical Pathology. pp. 15-20. ISSN 16185641 (ISSN)

[img] Text
Anti-leishmanial activities of selenium nanoparticles and selenium dioxide on Leishmania infantum.pdf

Download (303kB)

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Leishmania infantum is one of the important causes of visceral leishmaniasis in many countries. There are different complications for treatment of leishmaniasis such as toxicity and drug resistant. So far, there isn't any information about the effects of selenium nanoparticles and selenium dioxide (chemical form of selenium) on Leishmania parasites; hence, the aim of the present study is to investigate in vitro effects of six dilutions of these drugs on L. infantum. Anti-leishmanial activities were studied by adding different dilutions of 2.5, 5, 10, 25, 50, and 100 μg/ml of the drugs into promastigote cultures. Promastigote cytotoxicity was tested using the colorimetric MTT assay. Anti-amastigote activity was assessed in peritoneal macrophages of BALB/c mice. Also, cytotoxic effect of these drugs was evaluated on uninfected macrophages. The results showed that both of drugs have dose-dependent anti-leishmanial activities. Selenium NPs have more growth-inhibitory effect on promastigotes than SeO2; while the IC50 (50 inhibitory concentration) was determined to be 25 and 50 μg/ml, respectively. The mean numbers of amastigotes per macrophage in selenium NPs-treated groups were less than SeO2-treated and control groups. The IC50 of selenium NPs was 10 μg/ml and SeO2 was 25 μg/ml for amastigotes. Also, the IC50 of selenium NPs and SeO2 for uninfected macrophages were calculated to be 100 and 50 μg/ml, respectively. In addition, selenium NPs has less cytotoxic effect than SeO2 on uninfected macrophages. These findings suggest that selenium NPs have more anti-leishmanial properties and less cytotoxic effects than SeO2 against L. infantum. © 2012 Springer-Verlag London Limited.

Item Type: Article
Keywords: Anti-leishmanial activities Leishmania infantum Leishmaniasis Selenium dioxide Selenium nanoparticles
Divisions:
Page Range: pp. 15-20
Journal or Publication Title: Comparative Clinical Pathology
Journal Index: Scopus
Volume: 23
Number: 1
Identification Number: https://doi.org/10.1007/s00580-012-1561-z 10. 1155/2010/617521; Mohebali, M., Rezayat, M.M., Gilani, K., Sarkar, S., Akhoundi, B., Esmaeili, J., Satvat, T., Hooshyar, H., Nanosilver in the treatment of localized cutaneous leishmaniasis caused by Leishmania major (MRHO/IR
ISSN: 16185641 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/106

Actions (login required)

View Item View Item