Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

(2012) Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex. Iranian Journal of Microbiology. pp. 43-45. ISSN 20083289 (ISSN)

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Abstract

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.

Item Type: Article
Keywords: Mycobacterium tuberculosis Pulsed-filed gel electrophoresis TB-complex bacterial DNA article bacterium identification bacterium isolation clinical protocol DNA extraction nonhuman pulsed field gel electrophoresis Mycobacterium tuberculosis complex
Divisions:
Page Range: pp. 43-45
Journal or Publication Title: Iranian Journal of Microbiology
Journal Index: Scopus
Volume: 4
Number: 1
ISSN: 20083289 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/1135

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