Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Correlation of Hepatitis B surface antigen mutations with clinical status of the chronically infected patients from Kermanshah, West of Iran

(2012) Correlation of Hepatitis B surface antigen mutations with clinical status of the chronically infected patients from Kermanshah, West of Iran. Minerva Gastroenterol Dietol. pp. 9-18. ISSN 00264776 (ISSN)

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Abstract

Aim. Hepatitis B virus (HBV) genetic and protein variations have been found in chronic HBV infected patients who did not receive any treatment and active or passive immunizations. The aims of this study were to determine the genotypes as well as the patterns of variations distribution in chronically infected patients from the west part of Iran. Methods. Forty-six people with chronic HBV infection were enrolled in the study. The surface genes were amplified, sequenced and subsequently aligned using international and national Iranian database. Results. All strains belonged to genotype D, subgenotype D1 and subtype ayw2. Of all 116 "mutations" that occurred at 59 nucleotide positions, 49 (42.2) were missense (amino acid altering) and 67 (57.7) were silent (no amino acid changing), respectively. At the amino acid level, 38 (79-1) out of 48 amino acid mutations occurred in different immune epitopes within the surface proteins, of which 2 (5.2) occurred in B cell; 12 (31.5) in T helper and 24 (63.1) inside CTL epitopes. There were significant associations between amino acid mutations (especially within immune epitopes) and anti-HBe positivity and increased ALT levels (P values: 0.014 and 0.04, respectively). Conclusion. The distribution of amino acid mutations as well as the ratio between silent and missense nucleotide mutations showed that a narrowly focused immune pressure had already been on the surface protein T cell epitopes (94.9 of mutations), particularly CTL epitopes which led to the emergence of escape mutants in these patients.

Item Type: Article
Keywords: Amino acids Genotype Hepatitis B alanine aminotransferase amino acid hepatitis B surface antigen adult article B lymphocyte clinical article controlled study disease association female gene amplification gene mutation gene sequence genetic variability human immune response Iran male missense mutation nucleotide sequence phylogeny population genetics virus load Hepatitis B Surface Antigens Hepatitis B virus Hepatitis B, Chronic Humans Middle Aged Mutation Mutation, Missense
Divisions:
Page Range: pp. 9-18
Journal or Publication Title: Minerva Gastroenterol Dietol
Journal Index: Scopus
Volume: 58
Number: 1
ISSN: 00264776 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/1163

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