Abstract

Aims: Improvements in nanotechnology in the past decayed has created various opportunities for evaluation of biologic effects such as anti-bacterial effects of nanoparticles. The purpose of this study was to evaluate the antibacterial activity of calcium carbonate nanoparticles on two different bacteria including Agrobacterium tumefaciens and Staphylococcus aureus. Methods: The antibacterial effect of calcium carbonate nanoparticles against mentioned bacteria was evaluated by dilution in agar containing medium and dilution in Broth medium. Each of prepared Broth Media (10 ml) was inoculated with 1 ml of bacterial suspension (10 6 CFU/ml) and incubated. Sampling of culture Media was performed in specific intervals and diluted as 10 -1, to 10 -6. Then 100 μl of each sample was transferred to agar plates and was spread carefully and then incubated. Grown colonies were counted and MIC and MBC was determined. Results: Calcium carbonate nanoparticles showed very good antibacterial effect and after 16 hours the bacteria were totally diminished. The lowest and highest MIC concentration of these nanoparticles in solid medium was 31.2 and 125 μg/ml respectively. The MIC of calcium carbonate nanoparticles in Broth Medium was two times more than the MIC concentration in solid medium, while different concentrations of ordinary calcium carbonate not only revealed antibacterial effects but also supported the bacterial growth. Conclusion: Use of calcium carbonate nanoparticles as an anti-microbial agent is recommended in different fields of medicine, food industry and agriculture and can be of importance considering health and economic issues.