Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR

(2013) Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR. Int J Mol Cell Med. pp. 9-13. ISSN 2251-9637 (Print) 2251-9637

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/24551784

Abstract

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain.

Item Type: Article
Keywords: RT-qPCR Vibrio cholerae ctxAB expression
Divisions:
Page Range: pp. 9-13
Journal or Publication Title: Int J Mol Cell Med
Journal Index: Pubmed
Volume: 2
Number: 1
ISSN: 2251-9637 (Print) 2251-9637
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/1904

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