Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Reversal effect of intra-central amygdala microinjection of L-arginine on place aversion induced by naloxone in morphine conditioned rats

(2011) Reversal effect of intra-central amygdala microinjection of L-arginine on place aversion induced by naloxone in morphine conditioned rats. Iran Biomed J. pp. 92-9. ISSN 1028-852x

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/21987115

Abstract

BACKGROUND: Role of nitric oxide (NO) on expression of morphine conditioning using a solely classic task has been proposed previously. In this work, the involvement of NO on the expression of opioid-induced conditioning in the task paired with an injection of naloxone was investigated. METHODS: Conditioning was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Naloxone (0.05-0.4 mg/kg, i.p.), a selective antagonist of mu-opioid receptor, was administered once prior to morphine response testing. NO agents were administered directly into the central amygdala (CeA) prior to naloxone injection pre-testing. RESULTS: Morphine (2.5-10 mg/kg, s.c.) produced a significant dose-dependent place preference in experimental animals. When naloxone (0.05-0.4 mg/kg, i.p.) was injected before testing of morphine (5 mg/kg, s.c.) response, the antagonist induced a significant aversion. This response was reversed due to injection of L-arginine (0.3-3 microg/rat), intra-CeA prior to naloxone administration. However, pre-injection of L-NAME (intra-CeA), an inhibitor of NO production, blocked this effect. CONCLUSION: The finding may reflect that NO in the nucleus participates in morphine plus naloxone interaction.

Item Type: Article
Keywords: *Amygdala/drug effects/physiopathology Animals Arginine/*administration & dosage *Conditioning, Operant Dose-Response Relationship, Drug Male Microinjections Morphine/*administration & dosage Naloxone/*administration & dosage Narcotic Antagonists/*administration & dosage Rats Rats, Wistar Morphine Naloxone L-arginine L-name Place aversion
Divisions:
Page Range: pp. 92-9
Journal or Publication Title: Iran Biomed J
Journal Index: Pubmed
Volume: 15
Number: 3
ISSN: 1028-852x
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/2109

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