Abstract

Background As stress occurs repetitively every day, the biological modifiers should also have enough time to restore the normal state of hemostasis; otherwise, chronic stress would be anticipated. The aim of the present study was to examine the persistence of stress based on subjective emotion, salivary cortisol, and linear and non-linear features of heart rate variability (HRV) in both genders. Methods Thirty-three healthy young volunteers (23 men and 10 women) participating in this study were exposed to the Trier Social Stress Test (TSST). Moreover, the emotional visual analog scale (EVAS), salivary cortisol, and ECG recording in the rest state were taken before and after TSST as well as 20 min after recovery. Results According to the results of the two-way mixed model ANOVA, all volunteers showed a significant increase in EVAS after TSST which was restored to the baseline state after recovery. Notably, the women's base of cortisol was significantly higher than men and the standard range of kit. Cortisol elevation was only observed in the men, and the significant increase of LF/HF ratio was observed in the women, while both did not retain to the baseline after recovery. The SD1 of Poincare plot and spectral entropy decreased after stress in both genders. Moreover, there was a significant negative correlation between baseline level of cortisol and its elevation due to stress and some features of HRV. Conclusion The base of cortisol played a critical role in modifying the physiological response to stress. In addition, after recovery, no stressful emotion remained, while the non-linear features of HRV did not return to baseline.