Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Arginyl-glycyl-aspartic acid (RGD) containing nanostructured lipid carrier co-loaded with doxorubicin and sildenafil citrate enhanced anti-cancer effects and overcomes drug resistance

(2019) Arginyl-glycyl-aspartic acid (RGD) containing nanostructured lipid carrier co-loaded with doxorubicin and sildenafil citrate enhanced anti-cancer effects and overcomes drug resistance. Process Biochemistry. pp. 172-179. ISSN 1359-5113

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Abstract

Resistance to anticancer agents is considered as the main cause of chemotherapy failure. This study is aimed to prepare and optimize Doxorubicin (Dox) + Sildenafil citrate (SC)-coloaded Arginyl-glycyl-aspartic acid (RGD)-containing nanostructured lipid carriers (NLC-RGD) to overcome multidrug resistance limitation and improve cancer treatments. Consequently, DOX + SC-coloaded NLC-RGD were fabricated by homogenization method and characterized by several techniques. Then, cytotoxicity, cellular uptake, apoptosis, and expression level of some multi-drug resistance related genes were evaluated in DOX + SC-coloaded NLC-RGD treated cells. As results, particles with nano-size, narrow size distribution and suitable encapsulation efficiency (similar to 56% for DOX and similar to 81% for SC) were prepared. Our Results also demonstrated that co-delivery of DOX and SC by NLC-RGD promotes uptake and accumulation of drugs by integrin mediated endocytosis and possible ABC transporter inhibition. Cytotoxicity and apoptosis experiments revealed that co-delivery of DOX and SC by NLC-RGD is more effective approach for induction of apoptosis in comparison to individual treatment and delivery. Gene expression experiments revealed that SC reduces expression of ABCC1 and Nrf2. These findings indicated that NLC-RGD can be considered as an appropriate delivery system for co-delivery of DOX and SC to overcome DOX resistance to improve treatment efficacy in cancer.

Item Type: Article
Keywords: Doxorubicin Sildenafil citrate Cancer Nanostructured lipid carriers RGD delivery cancer nanoparticles paclitaxel Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Engineering
Divisions:
Page Range: pp. 172-179
Journal or Publication Title: Process Biochemistry
Journal Index: ISI
Volume: 84
Identification Number: https://doi.org/10.1016/j.procbio.2019.06.013
ISSN: 1359-5113
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/2410

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