Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Precise, direct, and rapid detection of Shigella Spa gene by a novel unmodified AuNPs-based optical genosensing system

(2019) Precise, direct, and rapid detection of Shigella Spa gene by a novel unmodified AuNPs-based optical genosensing system. Journal of Microbiological Methods. pp. 42-49. ISSN 0167-7012

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Abstract

Early detection of infectious bacteria is a necessity for combating infectious diseases. Due to low infectious dose of Shigella, rapid and sensitive detection is needed. Compared to the presented genes, Spa gene can be introduced as a novel sequence for all species of Shigella detection. Herein, the possibility of Spa genes for detection of four species of Shigella was investigated for the first time by AuNPs-based optical genosensing system. In this method, AuNP-DNA probes were hybridized with Spa gene sequence. When the complementary target is present, it prevents the aggregation of the complex under acid environment and the solution remains red whereas in the absence of the specific sequence, it turns to purple. Therefore, visual detection is possible with bare eye. The comparison of this Optical DNA biosensor and PCR-based method showed that the proposed method is simple, cost-effective, rapid operation, with high or comparable detection limit of (LOD and LOQ: 8.14 and 26.6 ng mLl(-1), respectively), without need of any expensive techniques, and equipments compared to the conventional methods. In conclusion, the described method may develop into a platform that could be utilized for detection of various bacterial species with high accuracy and prompt screening of samples.

Item Type: Article
Keywords: Optical Genosensor Shigella species Spa gene Unmodified gold nanoparticles real-time pcr multiplex pcr salmonella spp. antimicrobial resistance staphylococcus-aureus campylobacter-jejuni sensitive detection gold nanoparticles escherichia-coli clinical-samples Biochemistry & Molecular Biology Microbiology
Divisions:
Page Range: pp. 42-49
Journal or Publication Title: Journal of Microbiological Methods
Journal Index: ISI
Volume: 162
Identification Number: https://doi.org/10.1016/j.mimet.2019.05.007
ISSN: 0167-7012
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/2495

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