Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Specific fluorometric assay for direct determination of amikacin by molecularly imprinting polymer on high fluorescent g-C3N4 quantum dots

(2019) Specific fluorometric assay for direct determination of amikacin by molecularly imprinting polymer on high fluorescent g-C3N4 quantum dots. Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy. pp. 451-458. ISSN 1386-1425

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Abstract

Here, a specific and reliable fluorometric method for the rapid determination of amikacin was developed based on the molecularly imprinting polymer (MIP) capped g-C3N4 quantum dots (QDs). g-C3N4 QDs were obtained by facile and one-spot ethanol-thermal treatment of bulk g-C3N4 powder and showed a high yield fluorescence emission under UV irradiation. The MIP layer was also created on the surface on QDs, via usual self-assembly process of 3-aminopropyl triethoxysilane (APTES) functional monomers and tetraethyl ortho-silicate (TEOS) cross linker in the presence of amikacin as template molecules. The synthesized MIP-QDs composite showed an improved tendency toward the amikacin molecules. In this state, amikacin molecules located adjacent to the g-C3N4 QDs caused a remarkable quenching effect on the fluorescence emission intensity of QDs. This effect has a linear relationship with amikacin concentration and so, formed the basis of a selective assay to recognize amikacin. Under optimized experimental conditions, a linear calibration graph was obtained as the quenched emission and amikacin concentration, in the range of 3-400 ng mL(-1) (4.4-585.1 nM) with a detection limit of 1.2 ng mL(-1) (1.8 nM). The high selectivity of MIP sites as well as individual fluorescence properties of g-C3N4 QDs offers a high specific and sensitive monitoring method for drug detection. The method was acceptably applied for the measurement of amikacin in biological samples. (C) 2019 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: Amikacin G-C3N4 quantum dots Molecularly imprinting polymer Fluorescence human plasma aminoglycoside antibiotics spectrofluorometric method tungstate nanoparticles sensitive determination selective recognition sensor derivatization temperature oxide Spectroscopy
Divisions:
Page Range: pp. 451-458
Journal or Publication Title: Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy
Journal Index: ISI
Volume: 214
Identification Number: https://doi.org/10.1016/j.saa.2019.02.067
ISSN: 1386-1425
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/2564

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