Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products

(2019) Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products. Journal of Food Safety. p. 9. ISSN 0149-6085

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Abstract

Dairy products play a considerable role in transmission of Helicobacter pylori. The current study was done to assess phenotypic characters of antimicrobial resistance and genotyping pattern of vacA, cagA, and iceA alleles among the H. pylori strains isolated from dairy products. Eight-hundred dairy samples were collected and cultured. Antimicrobial resistance and genotyping patterns were assessed using disk diffusion and polymerase chain reaction, respectively. Thirty-one out of 800 (3.87) traditional dairy product samples were positive for H. pylori. Cheese (11.53) samples had the highest incidence of H. pylori, while yoghurt (1.11) had the lowest. Helicobacter pylori strains harbored the highest incidence of resistance against ampicillin (93.54), amoxicillin (93.54), tetracycline (90.32), erythromycin (80.64), and metronidazole (77.41). VacA s1a (90.32), m1a (90.32), s2 (80.64) and m2 (77.19), cagA (51.61), and iceA1 (32.25) were the most routinely identified genotypes. S1am1a (67.74), s2m1a (64.51), and s1am2 (51.61) were the most routinely identified genotyping pattern. Simultaneous presence of vacA, cagA, and iceA genotypes in antimicrobial resistant H. pylori strains indicates important public health issue regarding the consumption of dairy products. However, additional researches are required to find molecular genetic homology and other epidemiological aspects of H. pylori in dairy products. Practical applications Due to the high consumption rate of dairy samples, they should have a high microbial quality. Foods with animal origins and especially dairy samples are considered as a probable source of Helicobacter pylori infections. The incidence of contamination of dairy samples by H. pylori is fairly high, and nearly all of the isolates exhibited resistance against several types of antimicrobials and harbored pathogenic vacA, cagA, and iceA genotypes, so the risk of probable foodborne diseases caused by H. pylori in such products should not be neglected. Additionally, dairy samples may act as a reservoir of H. pylori with ability to transfer antimicrobial resistance and virulent genotypes.

Item Type: Article
Keywords: immuno sorbent assay antibiotic-resistance virulence factors isfahan province chicken meat bovine-milk glmm gene food transmission prevalence Biotechnology & Applied Microbiology Food Science & Technology
Divisions:
Page Range: p. 9
Journal or Publication Title: Journal of Food Safety
Journal Index: ISI
Volume: 39
Number: 2
Identification Number: https://doi.org/10.1111/jfs.12594
ISSN: 0149-6085
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/2631

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