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Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Enhanced antibacterial activity of imipenem immobilized on surface of spherical and rod gold nanoparticles

(2019) Enhanced antibacterial activity of imipenem immobilized on surface of spherical and rod gold nanoparticles. Journal of Physics D-Applied Physics. p. 7. ISSN 0022-3727

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Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that has a high resistance to antibiotics and can cause disease in patients with immune deficiency. Therefore, in the present study, the antimicrobial effects of imipenem-functionalized spherical and rod gold nanoparticles (SGNPs and RGNPs) have been investigated on P aeruginosa. Firstly, SGNPs and RGNPs were synthesized by citrate reduction and seed-growth methods, respectively. The SGNPs and RGNPs were conjugated with imipenem (Imp-SGNPs and Imp-RGNPs). The size, potential and morphology of the SGNPs and RGNPs were determined by different techniques including; TEM, DLS and UV-vis spectra. Different concentrations of Imp-SGNPs and Imp-RGNPs were used to determine the bacterial growth rate and minimum inhibitory concentration (MIC) by UV-vis spectrophotometry according to CLSI standard protocol. The results indicated that the drug loading on the surface of SGNPs and RGNPs was 60.5 and 54, respectively. The Imp-SGNPs and Imp-RGNPs exhibited greater antibacterial activity compared to that of imipenem alone. The highest antibacterial activity of GNPs was observed in the Imp-RGNPs against P aeruginosa. The MIC for 90 inhibition (MIC90) for imipenem, Imp-SGNPs and Imp-RGNPs was determined to be 43 mu g ml(-1), 21 mu g ml(-1) and 5.7 mu g ml(-1), respectively. These results indicated that the new nano-carrier systems with GNPs have great potential for enhanced antibacterial activities.

Item Type: Article
Keywords: immobilized antibacterial activity imipenem gold nanoparticles dna cytotoxicity Physics
Divisions:
Page Range: p. 7
Journal or Publication Title: Journal of Physics D-Applied Physics
Journal Index: ISI
Volume: 52
Number: 6
Identification Number: https://doi.org/10.1088/1361-6463/aaef4d
ISSN: 0022-3727
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/2707

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