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Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

The phylogenetic study of Escherichia coli strains isolated from clinical cases

(2016) The phylogenetic study of Escherichia coli strains isolated from clinical cases. Journal of Pure and Applied Microbiology. pp. 351-354. ISSN 09737510 (ISSN)

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Abstract

Urinary tract infection (UTI) is one of the common infectious diseases. Escherichia coli is one of the most important cause of UTI. The purpose of this research was to determine the frequency of some phylogenetic genes in E. coli strains isolated from patients with UTI. E. coli strains were recovered from urine culture of suspect patients with UTI and biochemical and bacterial tests have been utilized to identify the isolated bacteria. Detection of phylogenetic genes were done through Multiplex PCR. Approximately 94, 92, 82 and 72 of E. coli strains contained ChuA1, YjaA, TspE4C and arp genes respectively. Results indicate that PCR based phylogenetic molecular method is a simple and quick method for typing of our uropathogenic E. coli.

Item Type: Article
Keywords: PCR Phylogenic Uropathogenic E. coli amikacin ampicillin bacterial DNA cefotaxime ceftazidime ceftizoxime ceftriaxone ciprofloxacin cotrimoxazole gentamicin imipenem meropenem nalidixic acid nitrofurantoin norfloxacin ofloxacin adolescent adult aged antibiotic sensitivity Article bacterial genetics bacterial strain bacterium isolation DNA extraction Escherichia coli female genetic analysis human major clinical study male middle aged multiplex polymerase chain reaction nonhuman phylogeny polymerase chain reaction urinary tract infection urine culture
Divisions:
Page Range: pp. 351-354
Journal or Publication Title: Journal of Pure and Applied Microbiology
Journal Index: Scopus
Volume: 10
Number: 1
ISSN: 09737510 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/305

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