Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Using chitosan/polyethylen oxide (Cs/PEO) as a scaffold and wharton jelly-derived mesenchymal stem cell transplantation on the repair of transcected sciatic nerve in wistar rats: A behavioral and histological study

(2016) Using chitosan/polyethylen oxide (Cs/PEO) as a scaffold and wharton jelly-derived mesenchymal stem cell transplantation on the repair of transcected sciatic nerve in wistar rats: A behavioral and histological study. Journal of Mazandaran University of Medical Sciences. pp. 196-205. ISSN 17359279 (ISSN)

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Using chitosanpolyethylen oxide (CsPEO) as a scaffold and wharton jelly-derived mesenchymal stem cell transplantation on the repair of transcected sciatic nerve in wistar rats A behavioral and histological study.pdf

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Abstract

Background and purpose: The aim of this study was to determine the effects of chitosan/polyethylene oxide (Cs/PEO) scaffold and human mesenchymal stem cells derived from Wharton’s jelly (WJ-hMSCs) on regeneration of the sciatic nerve injury in rats. Materials and methods: In this research, 42 adult male rats were divided into six groups. 1Normal (intact) group: healthy rats without any nerve transection; 2- Control group: sciatic nerve was cut and sutured; 3- Sham group: culture medium was injected; 4- Cs/PEO group: scaffold with a thin Cs/PEO was used around the injury site; 5- WJ-hMSC group: WJ-hMSCs injected around the site of injury, and 6- WJ-hMSCs-Cs/PEO group: WJ-hMSCs were seeded on Cs/PEO membrane and used around the injury site. Behavioral assessments were done at 2, 4, 6 and 8 weeks following the surgery. Histological evaluation was performed at the end of the study. Results: Our results showed that behavioral values in experimental groups significantly increased when compared to intact group (P<0.05). These values in WJ-hMSC-Cs/PEO group significantly improved compared to the control and sham groups (P<0.05). In addition, histological findings revealed significant increase in the number of nerve fibers in experimental groups compared to intact group (P<0.05). Histological results revealed improvements in groups 4, 5 and 6, however, there was no significant difference between these groups and sham or control group. Conclusion: This study showed the positive effects of chitosan/polyethylene oxide scaffold and human mesenchymal stem cells derived from Wharton’s jelly on nerve regeneration of transected sciatic nerve in rat model. © 2016, AMazandaran University of Medical Sciences. All rights reserved.

Item Type: Article
Keywords: Chitosan scaffold Repair Sciatic nerve Wharton jelly chitosan macrogol adult animal experiment animal model animal tissue Article behavior assessment controlled study histology human human cell male mesenchymal stem cell transplantation nerve regeneration nonhuman rat sciatic nerve injury tissue engineering tissue scaffold
Divisions:
Page Range: pp. 196-205
Journal or Publication Title: Journal of Mazandaran University of Medical Sciences
Journal Index: Scopus
Volume: 25
Number: 133
ISSN: 17359279 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/323

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