Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Immunization with a recombinant fusion protein protects mice against Helicobacter pylori infection

(2018) Immunization with a recombinant fusion protein protects mice against Helicobacter pylori infection. Vaccine. pp. 5124-5132. ISSN 0264-410X

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More than 50 of the world's population is infected with the bacterium Helicobacter pylori. If left untreated, infection with H. pylori can cause chronic gastritis and peptic ulcer disease, which may progress into gastric cancer. Owing to the limited efficacy of anti-H. pylori antibiotic therapy in clinical practice, the development of a protective vaccine to combat this pathogen has been a tempting goal for several years. In this study, a chimeric gene coding for the antigenic parts of H. pylori FIiD, UreB, VacA, and CagL was generated and expressed in bacteria and the potential of the resulting fusion protein (rFUVL) to induce humoral and cellular immune responses and to provide protection against H. pylori infection was evaluated in mice. Three different immunization adjuvants were tested along with rFUVL: CpG oligodeoxynucleotides (CpG ODN), Addavax, and Cholera toxin subunit B. Compared to the control group that had received PBS, vaccinated mice showed significantly higher cellular recall responses and antigen-specific IgG2a, IgG1, and gastric IgA antibody titers. Importantly, rFUVL immunized mice exhibited a reduction of about three orders of magnitude in their stomach bacterial loads. Thus, adjuvanted rFUVL might be considered as a promising vaccine candidate for the control of H. pylori infection. (C) 2018 Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: Helicobacter pylori Immunization Chimeric Adjuvant Fusion protection brucella-melitensis infection vaccine ctb-ue confers protection therapeutic-efficacy provides protection oral immunization mucosal immunity gastric-cancer mouse model nod mice Immunology Research & Experimental Medicine
Page Range: pp. 5124-5132
Journal or Publication Title: Vaccine
Journal Index: ISI
Volume: 36
Number: 34
Identification Number:
ISSN: 0264-410X
Depositing User: مهندس مهدی شریفی

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