Abstract

Introduction: Synaptosomes are sealed particles that contain mitochondria, cytoskeleton and vesicles which are necessary to synaptic events like neurotransmitter release and uptake in the nervous system However, the effect of high and low temperatures on synaptosome membrane integrity and function during a time course after its extraction is less known The purpose of this study was to assess synaptosome viability and function at 37, 4 degrees C and room temperature (RT) during 6 hours after its extraction. Methods: Hippocampi of 40 male Wistar rats were used for synaptosome preparation To ensure synaptosome membrane integrity and function, lactate dehydrogenase activity (LDH) and GABA uptake were assessed during 6 successive hours after their extraction at 37, 4 degrees C and RT. Results: Our results showed that at 37 degrees C, synaptosome membrane integrity was reduced 3 hours but at 4 degrees C and RT, it occurred 5 hours following their extraction The results of synaptosome function analysis coincide with LDH enzyme assay data, meaning that GABA uptake faced a 50 reduction from the initial value at 37 degrees C after 3 hours and at RT after 5 hours We also found that GABA uptake was reduced at 4 degrees C in the first hour after extraction because the low temperature inhibits GABA transporters. Conclusion: Synaptosomes preserved their viability and function at RT, 37 and 4 degrees C at least for 3 hours after extraction and reduced over time For long term application of synaptosomes, it is better to keep them at 4 degrees C.