Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Nasal vaccination with r4M2e.HSP70c antigen encapsulated into N-trimethyl chitosan (TMC) nanoparticulate systems: Preparation and immunogenicity in a mouse model

(2018) Nasal vaccination with r4M2e.HSP70c antigen encapsulated into N-trimethyl chitosan (TMC) nanoparticulate systems: Preparation and immunogenicity in a mouse model. Vaccine. pp. 2886-2895. ISSN 0264-410X

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Nasal vaccination with r4M2e.HSP70c antigen encapsulated into N-trimethyl chitosan (TMC) nanoparticulate systems Preparation and immunogenicity in a mouse model.pdf

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Abstract

In this study, the potential of N-trimethyl chitosan (TMC) nanoparticles as a carrier system for the nasal delivery of the r4M2e center dot HSP70c, as an M2e-based universal recombinant influenza virus vaccine candidate, was investigated in mice. The anti-M2e specific cellular and humoral immune responses were assessed and the protective efficacy against a 90 lethal dose (LD90) of influenza A/PR/8/34 (H1N1) in a mice model was evaluated. Our results showed that the intranasal immunization of mice with r4M2e center dot HSP70c+TMC rather than the control groups, r4M2e+TMC, r4M2e and PBS (Phosphate buffer saline), significantly elevated both longevity and serum level of the total M2e-specific IgG antibody with a significant shift in the IgG2a/IgG1 ratio toward IgG2a, induced a Thl skewed humoral and cellular immune responses, increased IFN-gamma, IgG, and IgA in the bronchoalveolar lavage fluid (BALE), and promoted the proliferation of peripheral blood lymphocytes with lower morbidity and mortality rate against viral challenge. In conclusion, based on evidence to our finding, nasal vaccination with r4M2e center dot HSP70c antigen encapsulated into N-Trimethyl Chitosan (TMC) nanoparticulate system showed to induce a long lasting M2e-specific humoral and cellular immune responses and also provided full protection against a 90 lethal dose (LD90) of the influenza virus A/PR/8/34 (H1N1). It seems, protective immunity following intranasal administration of r4M2e could be resulted by the cooperation of both adjuvants, TMC and HSP70c. (C) 2018 Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: N-trimethyl chitosan (TMC) M2e HSP70 Intranasal Influenza Vaccine influenza-a virus immune-response fusion protein drug-delivery intranasal immunization extracellular domain universal vaccine m2 protein mucosal induction Immunology Research & Experimental Medicine
Divisions:
Page Range: pp. 2886-2895
Journal or Publication Title: Vaccine
Journal Index: ISI
Volume: 36
Number: 20
Identification Number: https://doi.org/10.1016/j.vaccine.2018.02.072
ISSN: 0264-410X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/3791

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