Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Electrochemical immunosensor for the breast cancer marker CA 15-3 based on the catalytic activity of a CuS/reduced graphene oxide nanocomposite towards the electrooxidation of catechol

(2018) Electrochemical immunosensor for the breast cancer marker CA 15-3 based on the catalytic activity of a CuS/reduced graphene oxide nanocomposite towards the electrooxidation of catechol. Microchimica Acta. p. 9. ISSN 0026-3672

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Abstract

The authors report on an electrochemical immunosensor for the tumor marker carbohydrate antigen 15-3 (CA15-3). It is based on the use of a composite consisting of reduced graphene oxide (RGO) and copper sulfide (CuS) that was placed on a screen-printed graphite electrode. The electrode shows excellent activity towards the oxidation of catechol acting as an electrochemical probe, best at a working potential of 0.16 V. The electrode was modified with antibody against CA15-3. Once the analyte (CA15-3) binds to the surface of the electrode, the response to catechol is reduced. The assay has a linear response in the 1.0-150 U mL(-1) CA15-3 concentration range, with a 0.3 U mL(-1) lower detection limit and a sensitivity of 1.88 mu A mu M-1 cm(-2). The immunosensor also shows good reproducibility (2.7), stability (95 of the initial values after storing for four weeks). The method was successfully applied to the determination of CA15-3 in serum samples, and results were found to compare well to those obtained by an ELISA. Conceivably, this nanocomposite based detection scheme has a wider scope and may be applied to numerous other immunoassays.

Item Type: Article
Keywords: Screen-printed electrode Copper sulfide Carbohydrate antigen 15-3 Tumor biomarkers Immunosensors Electrochemistry Biosensor tumor-necrosis-factor copper sulfide nanocrystals one-pot synthesis electrochemiluminescence immunosensor amperometric immunosensor ionic liquid factor-alpha electrode optimization immunoassays Chemistry
Divisions:
Page Range: p. 9
Journal or Publication Title: Microchimica Acta
Journal Index: ISI
Volume: 185
Number: 1
Identification Number: https://doi.org/10.1007/s00604-017-2532-5
ISSN: 0026-3672
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/3980

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