Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Effect of Physical and Chemical Factors in Production of Alkaline Protease Enzyme by Bacillus Strains

(2018) Effect of Physical and Chemical Factors in Production of Alkaline Protease Enzyme by Bacillus Strains. International Letters of Natural Sciences. pp. 10-16. ISSN 2300-9675

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Abstract

Proteases is family of enzymes and it has crucial role due to their physiological roles and very valuable commercial applications. Alkaline protease are produced by Bacillus species are particular importance because of their thermal stability and stability at different pH values. This study aimed to investigate the effect of physical and chemical factors in production of alkaline protease enzyme fermentation by members of the genus Bacillus. In this study, alkaline protease enzyme production were evaluated in submerged fermentation by Bacillus strains which were isolated from alkaline soils of Guilan province. Factors incubation were optimized such as time, pH, amount of inoculation and ammonium sulfate in alkaline protease enzyme production whit using response surface methodology (RSM) in culture. The maximum enzymatic activity was observed in incubation time of 36 hours, pH=9, inoculation amount of 15 (V) and ammonium sulfate 1.5 (W/V). Factors had significant effect on the production of alkaline protease enzyme such as pH and ammonium sulfate.

Item Type: Article
Keywords: Alkaline protease Bacillus RSM optimization parameters Science & Technology - Other Topics
Divisions:
Page Range: pp. 10-16
Journal or Publication Title: International Letters of Natural Sciences
Journal Index: ISI
Volume: 71
Identification Number: https://doi.org/10.18052/www.scipress.com/ILNS.71.10
ISSN: 2300-9675
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4002

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