Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

The prevalence of quinolone resistance genes of A, B, S in Escherichia coli strains isolated from three major hospitals in Tehran, Iran

(2018) The prevalence of quinolone resistance genes of A, B, S in Escherichia coli strains isolated from three major hospitals in Tehran, Iran. Central European Journal of Urology. pp. 129-133. ISSN 2080-4806

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Abstract

Introduction Escherichia coli (E.coli) as an opportunistic pathogen is a major cause of the hospital infections. The main goal of this research was to determine the frequency of quinolone resistance genes (qnr) among E. coli pathotypes isolated from patients with urinary tract infections (UTIs). Material and methods Urine samples were obtained from patients with UTIs in three major hospitals of Mofid, Bu Ali, and Vali-Asr during the year of 2015 in Tehran, Iran. The antibiogram was done for isolated bacterial isolates using nalidixic acid, norfloxacin, gentamicin, streptomycin, and chloramphenicol. Then the plasmids of the bacterial samples were extracted. PCR was used to detect qnr genes. Finally, the PCR products were run on a 1 agarose gel electrophoresis and the results were analyzed by the program SPSS version 22. Results Overall, 100 E. coli strains were isolated from patients with UTIs. The highest resistance rate was against Streptomycin. The frequency of the genes of qnrA, qnrB and qnrS were 0, 25 and 36, respectively. Moreover, the presence of the both genes of qnrB and qnrS was recognized in 10 of isolated bacterial strains. Conclusions Our results indicated increasing rates of quinolone resistant E. coli strains circulating in hospitals under the study. Dissemination of these strains harboring qnr determinants is of parti-cular concern.

Item Type: Article
Keywords: Escherichia coli qnr urinary tract infections antibiotic resistance urinary-tract-infections pediatric-patients virulence factors susceptibility receptors Urology & Nephrology
Divisions:
Page Range: pp. 129-133
Journal or Publication Title: Central European Journal of Urology
Journal Index: ISI
Volume: 71
Number: 1
Identification Number: https://doi.org/10.5173/ceju.2018.1539
ISSN: 2080-4806
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4030

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