(2015) Vaginal lactobacilli and pap operon expression in uropathogenic Escherichia coli. Biomedical and Pharmacology Journal. pp. 91-97. ISSN 09746242 (ISSN)
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Vaginal lactobacilli and pap operon expression in uropathogenic Escherichia coli.pdf Download (79kB) |
Abstract
The present study aims to evaluate the effects of lactobacillus by-products on pyelonephritis-associated pili pap expression in Escherichia (E.) coli. Vaginal lactobacillus rhamnosus and lactobacillus crispatus species were isolated and the effects of their culture supernatants (CS) on pap promoter activity in uropathogenic E. coli (UPEC) were studied. A new reporter construct of pET28a including Iranian firefly luciferase coding sequence was used to assess the pap propotor activity. The lactobacillus rhamnosus forced the pap promoter to turn off when UPEC was exposed to 1, 1.1, and 1.3 of the culture supernatant. In addition, lactobacillus crispatus could continuously switch off pap promoter phase variations when UPEC was exposed to a serial dilution of culture supernatant. The findings showed therapeutic and preventive potential of lactobacillus rhamnosus and lactobacillus crispatus for bacterial vaginitis and urinary tract infection (UTI) through controlling the virulence factors expression via their ecological origins.
| Item Type: | Article |
|---|---|
| Keywords: | Escherichia coli Lactobacillus crispatus Lactobacillus rhamnosus Urinary Tract Infection Vaginitis |
| Divisions: | |
| Page Range: | pp. 91-97 |
| Journal or Publication Title: | Biomedical and Pharmacology Journal |
| Journal Index: | Scopus |
| Volume: | 8 |
| Identification Number: | https://doi.org/10.13005/bpj/563 |
| ISSN: | 09746242 (ISSN) |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.bmsu.ac.ir/id/eprint/419 |
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