Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Neural differentiation of human induced pluripotent stem cells on polycaprolactone/gelatin bi-electrospun nanofibers

(2017) Neural differentiation of human induced pluripotent stem cells on polycaprolactone/gelatin bi-electrospun nanofibers. Materials Science & Engineering C-Materials for Biological Applications. pp. 1195-1202. ISSN 0928-4931

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Abstract

In the present study, for the first time, polycaprolactone (PCL) and gelatin (GEL) were used for neural differentiation of human induced pluripotent stem cells (hiPSCs) in the form of bi-electrospun nanofibers. The electrospun fibers were evaluated by FTIR and tensile analysis. MIT assay was used to evaluate the toxicity on the scaffolds. The hiPSCs were seeded on the fibers and after 14 days in neural differentiation medium. To confirm the differentiation, real-time PCR and immunocytochemistry (ICC) analyses were performed. For morphological studies of fibers and cultured cells on them, scanning electron microscopy (SEM) and optical microscopy (OM) were used. Our results indicated that hiPSCs had differentiated to neural cells completely after incubation time. Our study demonstrates that PCL/GEL bi-electrospun nanofibers not only have the capability to support hiPSCs differentiation to neural cells, but they also are able to enhance and improve such process. Overall, PCL/GEL scaffolds seem to be a feasible, reliable and easily accessed composite for further tissue engineering experiments. (C) 2017 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: Human induced pluripotent stem cells (hiPSCs) Neural differentiation Tissue engineering Scaffold Polycaprolactone (PCL) Gelatin (GEL) tissue engineering applications spinal-cord-injury fibrous scaffolds mechanical-properties in-vitro gelatin nerve fabrication fibers poly(l-lactide-co-epsilon-caprolactone) Materials Science
Divisions:
Page Range: pp. 1195-1202
Journal or Publication Title: Materials Science & Engineering C-Materials for Biological Applications
Journal Index: ISI
Volume: 78
Identification Number: https://doi.org/10.1016/j.msec.2017.04.083
ISSN: 0928-4931
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4289

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