Abstract

The Trachyspermum ammi plant has anti-fungal properties. The aim of this study is to examine the anti-fungal effects of this plant on the pro-inflammatory biomarkers, which are important in the course of fungal diseases. At first the ethanol extract of the Trachyspermum ammi plant was prepared from the Iranian biological resource center. We used monocyte/macrophage cells as samples because they were similar to the cells with fungal infection. Then THP-1 monocyte/macrophages (5×105 cells/well) were incubated at a humidity of 90, 5 CO2, 37UC for 72 h with control media alone or Trachyspermum ammi at concentrations of 10 ?g/ml. One set of cells was activated for 1 h with mannose (3 mg/ml) for both reverse-transcriptase Polymerase chain reaction and real-time Polymerase chain reaction analysis of Tumor necrosis factor alpha (TNF-α), Interleukin-18 (IL-18) expression. The activated cells by mannose with a plant extract redused the Interleukin-18 and Tumor necrosis factor alpha expression as activated cell by mannose with fluconazole (p-value=0.91 for IL-18, p-valu=0.25 for TNF-α). Based on the PCR and realtime methode to analysis of gene expression, Trachyspermum ammi plant such as the flavonoids has noticeable anti-fungal properties.