Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Design and analysis of trivalent chimeric vaccine candidate against three enterotoxigenic bacteria: an in-silico approach

(2017) Design and analysis of trivalent chimeric vaccine candidate against three enterotoxigenic bacteria: an in-silico approach. Minerva Biotecnologica. pp. 62-75. ISSN 1120-4826

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Abstract

BACKGROUND: Diarrheal diseases caused by enteric bacteria remain as a leading cause of preventable death, especially among children under five years old in developing countries. In these cases, vaccines could be considered as an important solution for prevention of these diseases. The toxins of Vibrio cholerae (CT), Shiga-toxin-producing Escherichia coli (Stx) and enterotoxigenic E. coli ( LT) are the most important virulent factors of these three enteric pathogens. In this study, we designed a trivalent recombinant immunogene from non-toxic part of these virulence factors consists of LtB (L), StxB (S) and CtxB (C). This chimeric protein (LSC) carrying important epitopes and the sequences with adjuvant potential activity, which could increase the possibility of eliciting effective immune responses. METHODS: For successful heterologous expression of the LSC sequence, the genetic codes and a variety of critical parameters in the synthetic gene were optimized. Thermodynamic analyses for folding of mRNA structures revealed correct folding of the RNA representative good stability of this molecule. In-silico analysis was carried out to predict three-dimensional structure of this hypothetical protein and the model was validated using Ramachandran plot analysis. RESULTS: High antigenicity of the construct was predicted by immunoinformatic analysis. Continuous and discontinuous B-cell epitopes were identified and showed the proper immunogenicity of this multimeric recombinant protein. CONCLUSIONS: The results suggested that the protein deduced from this construct could act as a vaccine candidate against three important causative agents of diarrheal bacteria.

Item Type: Article
Keywords: Vaccines E. coli heat-labile enterotoxin Shiga toxins Cholera toxin Computer simulation toxin-b-subunit enterohemorrhagic escherichia-coli heat-labile enterotoxin subcellular-localization neutralizing antibodies web server protein prediction expression cholera Biotechnology & Applied Microbiology
Divisions:
Page Range: pp. 62-75
Journal or Publication Title: Minerva Biotecnologica
Journal Index: ISI
Volume: 29
Number: 2
Identification Number: https://doi.org/10.23736/s1120-4826.16.01997-2
ISSN: 1120-4826
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4413

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