Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Targeted DNA delivery to cancer cells using a biotinylated chitosan carrier

(2017) Targeted DNA delivery to cancer cells using a biotinylated chitosan carrier. Biotechnology and Applied Biochemistry. pp. 423-432. ISSN 0885-4513

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Abstract

A novel biotinylated chitosan-graft-polyethyleneimine (Bio-Chi-g-PEI) copolymer was synthesized and evaluated as a nonviral gene delivery carrier for improvement of the transfection efficiency, endosomal escape, and targeted gene delivery of a plasmid encoding green fluorescent protein N1 (pEGFP-N1) into two different biotin-overexpressing cell lines including HeLa and OVCAR-3 cells. The structure of the obtained copolymers was confirmed by H-1 nuclear magnetic resonance (H-1 NMR) and Fourier transform infrared spectroscopy. Physicochemical properties of the Bio-Chi-g-PEI/plasmid DNA (pDNA) complexes such as complex stability, size, zeta potential, and their morphology were investigated at various weight ratios of copolymer to pDNA. Bio-Chi-g-PEI copolymers could effectively condense pDNA into small particles with average diameters less than 164 nm and the zeta potential of + 34.8 mV at the N/P ratio of 40/1. As revealed by flow cytometry, Bio-Chi-g-PEI/pDNA complexes had lower cytotoxicity than that of PEI 25 kDa/ pDNA complexes in both cell lines. In vitro experiments revealed that the Bio-Chi-gPEI/pDNA complexes not only had much lower cytotoxicity, but also displayed higher transfection efficiency than that of PEI 25kDa/pDNA complexes. High percentage of cancer cells was successfully transfected by Bio-Chi-g-PEI/pDNA and properly expressed GFP protein. This study indicates that this copolymer complex can be a promising gene delivery carrier. (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.

Item Type: Article
Keywords: chitosan linked PEI copolymer synthesis DNA delivery gene nanocarriers nanoparticle nonviral vectors low-molecular-weight transfection efficiency gene delivery in-vitro nonviral vector physicochemical properties carcinoma-cells polyethylenimine nanoparticles vivo Biochemistry & Molecular Biology Biotechnology & Applied Microbiology
Divisions:
Page Range: pp. 423-432
Journal or Publication Title: Biotechnology and Applied Biochemistry
Journal Index: ISI
Volume: 64
Number: 3
Identification Number: https://doi.org/10.1002/bab.1497
ISSN: 0885-4513
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4430

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