Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens

(2017) BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens. Iranian Journal of Immunology. pp. 123-133. ISSN 1735-1383

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Abstract

Background: Echinococcosis is a zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. Several native and recombinant antigens, derived from different stages of E. granulosus life cycle, have been used for vaccine trials. In vitro reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for researchers. Objective: To evaluate different antigens derived from in vitro reared E. granulosus adult worms for the immunization of BALB/c mice against secondary hydatidosis. Methods: Viable protoscoleces (PCSs) of sheep hydatid cyst were cultivated in S. 10E.H media. Excretory secretory (E/S) and crude antigens were prepared from reared adult worms. A total of fifty BALB/c mice, each 8-weeks-old, were divided into 5 groups of 10 mice. Three groups were subcutaneously immunized with crude, E/S and immunodominant antigens on days 1 and 28. The fourth group received only PBS and the fifth group had no injection. Three weeks following the second immunization, all groups were challenged, intraperitoneal, with viable PSCs. After the autopsy of the mice and opening their abdominal wall, cysts were counted and measured followed by histopathological observations. Results: The highest protective immunity (98.7) against hydatidosis was induced by crude antigen, followed by E/S and immunodominant antigens. Conclusion: Antigens (crude antigens in particular) derived from in vitro reared E. granulosus adult worms, and their different protein components are suitable candidates for the vaccination of intermediate hosts against hydatidosis.

Item Type: Article
Keywords: In vitro Echinococcus granulosus Protective immunity Hydatidosis BALB/c human cystic echinococcosis host mouse model recombinant antigen vaccination protoscoleces disease sheep protection antibody humans Immunology
Divisions:
Page Range: pp. 123-133
Journal or Publication Title: Iranian Journal of Immunology
Journal Index: ISI
Volume: 14
Number: 2
ISSN: 1735-1383
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4506

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