(2017) Bioinformatic prediction and experimental validation of a PE38-based recombinant immunotoxin targeting the Fn14 receptor in cancer cells. Immunotherapy. pp. 387-400. ISSN 1750-743X
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Abstract
Aim: AFn14R can serve as an ideal target for cancer immunotherapy. Here, a combined bioinformatic and experimental approach was applied to characterize an immunotoxin consisting of single-chain variable fragment antibody that targets Fn14 and a toxin fragment (PE38). Methods & Results: Flow cytometry results showed that the rate of PE38-P4A8 binding to Fn14 was approximately 60 and 40 in HT-29 and A549 cells, respectively. Moreover, 1 ng/mu l of immunotoxin was able to lyse approximately 53 and 41 of HT-29 and A549, respectively. PE38-P4A8 showed stability in mouse serum (similar to 90) after 3-h incubation. Most importantly, using bioinformatics for determining the structure and function of fusion proteins can be very helpful in designing of experiments. Conclusion: Coupled with bioinformatics, experimental approaches revealed that PE38-P4A8 could be used as a promising therapeutic agent for cancer cells expressing Fn14.
| Item Type: | Article |
|---|---|
| Keywords: | cancer Fn14R immunogenicitiy immunotoxin inclusion-body proteins ricin-a-chain pseudomonas exotoxin in-vitro polyethylene-glycol antitumor-activity low immunogenicity codon usage identification antibodies Immunology |
| Divisions: | |
| Page Range: | pp. 387-400 |
| Journal or Publication Title: | Immunotherapy |
| Journal Index: | ISI |
| Volume: | 9 |
| Number: | 5 |
| Identification Number: | https://doi.org/10.2217/imt-2017-0008 |
| ISSN: | 1750-743X |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.bmsu.ac.ir/id/eprint/4531 |
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