Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Multiplex PCR for detection of water-borne bacteria

(2017) Multiplex PCR for detection of water-borne bacteria. Water Science and Technology-Water Supply. pp. 169-175. ISSN 1606-9749

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Abstract

Microbial water-borne diseases still affect developing countries and are major water quality concerns throughout the world. Routine culture-based methods of identifying bacterial pathogens in water sources are laborious and time-consuming. Recently, the use of molecular techniques such as the polymerase chain reaction (PCR) has provided rapid and highly promising detection methods. In this study, we developed two multiplex PCR assays for simultaneous detection of six water-borne bacteria. Two triplex PCR protocols were developed to detect six target genes. The first protocol targets uidA (Escherichia coli), int (Shigella spp.), and gyrB (Pseudomonas aeruginosa) genes, while invA (Salmonella spp.), ompW (Vibrio cholera), and lacZ (coliforms) were amplified by the second protocol. Specificity testing was carried out for 12 reference strains. Furthermore, the applicability of the multiplex PCR assays for detection of these bacteria was investigated for 52 surface water samples. The results indicated that all primer pairs showed specificities only for their corresponding target organisms. The detection sensitivity of both multiplex PCR assays was 3 x 10(2) 3 x 10(3) colony forming units. The developed assays represent simple and efficient diagnostic procedures for co-detection of water-borne bacteria and have the potential to provide earlier warnings of possible public health threats and more accurate surveillance of these organisms.

Item Type: Article
Keywords: Escherichia coli multiplex PCR Pseudomonas aeruginosa Salmonella spp. Shigella spp. Vibrio cholerae water rapid detection escherichia-coli pathogens assay quantification salmonella Engineering Environmental Sciences & Ecology Water Resources
Divisions:
Page Range: pp. 169-175
Journal or Publication Title: Water Science and Technology-Water Supply
Journal Index: ISI
Volume: 17
Number: 1
Identification Number: https://doi.org/10.2166/ws.2016.126
ISSN: 1606-9749
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4568

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