Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Assessment of Neuroprotective Properties of Melissa officinalis in Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury

(2016) Assessment of Neuroprotective Properties of Melissa officinalis in Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury. ASN Neuro. p. 17. ISSN 1759-0914

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Abstract

Introduction: The pathophysiology of spinal cord injury (SCI) has a classically bad prognosis. It has been demonstrated that human umbilical cord blood stem cells (hUCBSCs) and Melissa officinalis (MO) are useful for the prevention of neurological disease. Methods: Thirty-six adult male rats were randomly divided into intact, sham, control (SCI), MO, hUCBSC, and MO-hUCBSC groups. Intraperitoneal injection of MO (150 mg/kg) was commenced 24 hr post-SCI and continued once a day for 14 days. Intraspinal grafting of hUCBSCs was commenced immediately in the next day. The motor and sensory functions of all animals were evaluated once a week after the commencement of SCI. Electromyography (EMG) was performed in the last day in order to measure the recruitment index. Immunohistochemistry, reverse transcription-polymerase chain reaction, and transmission electron microscopy evaluations were performed to determine the level of astrogliosis and myelination. Results: The results revealed that motor function (MO-hUCBSC: 15 +/- 0.3, SCI: 8.2 +/- 0.37, p < .001), sensory function (MO-hUCBSC: 3.57 +/- 0.19, SCI: 6.38 +/- 0.23, p < .001), and EMG recruitment index (MO-hUCBSC: 3.71 +/- 0.18, SCI: 1.6 +/- 0.1, p < .001) were significantly improved in the MO-hUCBSC group compared with SCI group. Mean cavity area (MO-hUCBSC: 0.03 +/- 0.03, SCI: 0.07 +/- 0.004, p < .001) was reduced and loss of lower motor neurons (MO-hUCBSC: 7.6 +/- 0.43, SCI: 3 +/- 0.12, p < .001) and astrogliosis density (MO-hUCBSC: 3.1 +/- 0.15, SCI: 6.25 +/- 1.42, p < 0.001) in the ventral horn of spinal cord were prevented in MO-hUCBSC group compared with SCI group. Conclusion: The results revealed that the combination of MO and hUCBSCs in comparison with the control group has neuroprotective effects in SCI.

Item Type: Article
Keywords: astrogliosis human umbilical cord blood Melissa officinalis myelination spinal cord injury functional recovery neuronal apoptosis oxidative stress wistar rats transplantation extract behavior model mice l. Neurosciences & Neurology
Divisions:
Page Range: p. 17
Journal or Publication Title: ASN Neuro
Journal Index: ISI
Volume: 8
Number: 6
Identification Number: https://doi.org/10.1177/1759091416674833
ISSN: 1759-0914
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/4800

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