Abstract

Acinetobacter species particularly Acinetobacter baumannii (A. baumannii) have been widely reported as broad-spectrum antibiotic resistant pathogens. Expression of various types of metallo beta-lactamases (MBL), classified as Ambler class B, has been associated with carbapenem resistance. Here, we attempted to assess the frequency of extensively drug-resistant (XDR) and MBL-producing A. baumannii among clinical isolates. 86 clinical A. baumannii strains were collected from 2014 to 2015 and their susceptibility to meropenem (10 mu g), imipenem (10 mu g), azteronem (30 mu g), pipracillin (100 mu g) tazobactam (110 mu g), tobramycin (10 mu g), fosfomycin (200 mu g), rifampicin (5 mu g), colistin (10 mu g), tigecycline (15 mu g), sulbactam/ampicillin (10 mu g + 10 mu g) and polymixin B (300 U) was evaluated using disk diffusion method. The MBL-producing isolates were screened using combined disc diffusion method. Furthermore, the presence of bla(VIM), bla(IMB), bla(SPM), bla(GIM), bla(SIM) and bla(NDM) was detected by PCR. 34.9 of isolates were recovered from bronchoalveolar lavage (BAL). 81 (94.2) and 62 (71.2) isolates were multidrug resistance (MDR) and XDR, respectively. 44 (51.2) and 65 (75.6) isolates were MBL-producing strains with resistance to imipenem and meropenem, respectively. 2 (23), 13 (15.1), 2 (2.3), 4 (4.7) and 2 (23) isolates carried bla(VIM), bla(IMB), bla(SPM), bla(GIM), and bla(SIM) genes, respectively. Our data showed that the rate of XDR and MBL A. baumannii is on the rise. (C) 2016 Elsevier Ltd. All rights reserved.