Abstract

The aim of this study is to analyze oxidative stress (OS) and changes in expression of reactive oxygen species (ROS) producing-related genes in mustard lungs. Human lung biopsies provided from controls (n =5) and sulfur mustard (SM)-exposed patients (n= 6). Changes in expression of dual oxidases (DUOXs), aldehyde oxidase 1 (AOX1), thyroid peroxidase (TPO), myeloperoxidase (MPO) and eosinophil Peroxidase (EPO) were measured using RT2 Profiler (TM) PCR Array. OS was evaluated by determining bronchoalveolar lavage fluids (BALF) levels of total antioxidant capacity (TAC) and malondialdehyde (MDA). Higher TAC value was observed in BALF of controls compared with patients (0.138 +/- 1 0.02683 mu mol/l vs 0.0942 +/- 0.01793 mu mol/l), whereas a significant increase in MDA concentration was found in patients (0.486 +/- 0.04615 nmol/l vs 0.6467 +/- 0.05922 nmol/l). All ROS producing-related genes were overexpressed in the order AOX1 > MPO > DUOX2> DUOX1> TPO> EPO. Upregulation of these genes may be a reason for overproduction of ROS, oxidants/antioxidants imbalance, OS and respiratory failures in mustard lungs. (C) 2016 Published by Elsevier B.V.