Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Design and in silico analysis of pentavalent chimeric antigen against three enteropathogenic bacteria: enterotoxigenic E. coli, enterohemorragic E. coli and Shigella

(2016) Design and in silico analysis of pentavalent chimeric antigen against three enteropathogenic bacteria: enterotoxigenic E. coli, enterohemorragic E. coli and Shigella. Bioscience Biotechnology Research Communications. pp. 229-243. ISSN 0974-6455

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Design and in silico analysis of pentavalent chimeric antigen against three enteropathogenic bacteria enterotoxigenic E. coli, enterohemorragic E. coli and Shigella.pdf

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Abstract

Astonishing improvements in information technology, in combination with the valuable experimental data gathered during the past decades, has revolutionized vaccine design strategies. On the other hand, the development of genetic engineering methods has enabled us to design and produce new chimeric proteins for many different purposes, including immunization. In this study we took the advantages of these improvements to develop an efficacious vaccine against three important enteropathogenic bacteria: enterotoixigenic E. coli (ETEC), enterohemorragic E. coli (EHEC), and Shigella. To do this, appropriate immunogens, including subunit B of heat labile toxin and mutated STa toxin from ETEC; EspA and Stx B from EHEC; and the N-terminal part of IpaD, an immunogen from diffrent Shigella strains, were selected. These proteins were fused together by an appropriate peptide linker. Bioinformatic analyses, including the physicochemical parameters calculation, secondary and tertiary structures prediction and verification, antigenic B-cell and T-cell epitopes prediction were performed. Then the protein was reverse-translated to DNA and was codon optimized for expression in E. coli cells. The results showed a proper 3D structure, good theoretical immunogenicity, invitro and invivo stability of the chimeric designed protein and proper structure and stability of the related mRNA. Altogether, the results suggest that the designed chimeric protein, structurally and immunologically, has almost all factors of an efficient vaccine candidate and can be tested in experimental studies.

Item Type: Article
Keywords: chimeric antigen in silico analysis candidate vaccine design etec ehec shigella protein secondary structure heat-stable enterotoxin escherichia-coli web server virulence factors fusion protein prediction toxin vaccines infections Biotechnology & Applied Microbiology
Divisions:
Page Range: pp. 229-243
Journal or Publication Title: Bioscience Biotechnology Research Communications
Journal Index: ISI
Volume: 9
Number: 2
Identification Number: https://doi.org/10.21786/bbrc/9.1/9
ISSN: 0974-6455
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5035

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