Abstract

Introduction: In recent years a great deal of effort has been made to improve the culture medium which can be used for different cell culturing and Folliculogenesis. PL (Platelet Layset) with a high percentage of growth factors and also microelements can be effective on the growth of follicle and oocyte. Material and method: Preantral follicles were collected from 12 to 14 days female NMRI mice and cultured in culture medium with a different supplement as the serum for 12 days. The different serums were, 5 and 10 FBS as the control groups and the experimental groups were enriched by 5 PL, 10 PL and a combination of 5 PL and 5 FBS. The growth and development of follicle and oocyte was monitored and also the amount of Estradiol (E2) and progesterone (P4) was detected in different mediums on days 9 and 12 of culturing. Result: Oocytes had a significant growth in all medium compared to day zero and reached to the size of a mature oocyte (p < 0.05). Although survival rate after one day had significant decrease in all medium compared to 5 FBS (p < 0.05), after 12 days of culturing, the best survival rate belonged to 5 PL. Progesterone secretion increased in experimental groups significantly (p < 0.05) while Estradiol secretion in 5 and 10 PL decreased in half of control groups. Discussion: This result demonstrates that PL can be a considerable supplement that can be added to the culture medium or a good replacement for FBS as serum used in Folliculogenesis. (C) 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of Middle East Fertility Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).