Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Time Profile of nNOS Expression in the Spinal Dorsal Horn after L-5 Spinal Root Transection in Rats

(2015) Time Profile of nNOS Expression in the Spinal Dorsal Horn after L-5 Spinal Root Transection in Rats. Neurophysiology. pp. 287-294. ISSN 0090-2977

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Abstract

Using immunohistochemical analysis, we investigated the time profile of neuronal nitric oxide synthase (nNOS) expression in the lumbar spinal cord up to day 28 after transection of the L-5 spinal root. On day 14 after injury, we also evaluated the effect of intrathecal application of 7-nitroindazole (7-NI), a selective nNOS inhibitor (8.15 mu g in 5 mu l), on thermal hyperalgesia. Our results indicated that nerve transection increased the intensity of nNOS-immunoreactivity in superficial and deep laminae of the dorsal horn within a late stage (days 7 to 28) of the neuropathy model used. Furthermore, 7-NI attenuated nerve injury-evoked thermal hypersensitivity on day 14 but did not reduce it between days 2 and 5 after transection. These data suggest that nNOS overexpression is more involved in the development than in the initiation of thermal hyperalgesia in L-5-transected rats.

Item Type: Article
Keywords: neuropathic pain spinal dorsal horn L-5 segment nNOS 7-nitroindazole thermal hyperalgesia nitric-oxide synthase sciatic-nerve transection neuropathic pain messenger-rna cord immunoreactivity mechanisms ganglion model jun Neurosciences & Neurology Physiology
Divisions:
Page Range: pp. 287-294
Journal or Publication Title: Neurophysiology
Journal Index: ISI
Volume: 47
Number: 4
Identification Number: https://doi.org/10.1007/s11062-015-9535-9
ISSN: 0090-2977
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5401

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