Abstract

Background: Wound healing and other tissue abnormalities are considered as major concerns in diabetes. The novel therapeutic options are urgently needed for treatment of infections in diabetes caused by multidrug resistant S. aureus. Reportedly, HAMLET could sensitize bacterial pathogens to conventional antimicrobial agents. The objective of the present study was to evaluate wound healing activity of methicillin in presence of HAMLET in methicillin resistant Staphylococcus aureus infected wounds in diabetic rats. Materials & Methods: Fifty male diabetic rats were randomized into five groups of ten animals each. Induction of diabetes was achieved using 60 mg/kg streptozotocin. In group I, 0.1 mL sterile saline 0.9 solution was added to the wounds with no infection. In group II, the wounds were infected with MRSA and only treated with 0.1 mL the sterile saline 0.9 solution. In group III, infected wounds were treated with HAMLET (100 μg). In group IV, animals with infected wounds were treated with 0.1 mL topical application of 1 mg/mL methicillin. In group V, animals with infected wounds were treated with topical application of 0.1 mL solution of methicillin (1 mg/mL) and HAMLET (100 μg). Results: Microbiological examination, planimetric, histological and quantitative morphometric studies and determination of hydroxyproline levels showed that there was significant difference between animals in group V compared to other groups (P=0.001). Conclusions: HAMLET could make methicillin useful for treatment of MRSA infected wounds in diabetes. © 2019 Revista Latinoamericana de Hipertension. All rights reserved.