Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Downregulation of external death receptor genes FAS and DR5 in colorectal cancer samples positive for human papillomavirus infection

(2015) Downregulation of external death receptor genes FAS and DR5 in colorectal cancer samples positive for human papillomavirus infection. Pathology Research and Practice. pp. 444-448. ISSN 0344-0338

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Abstract

Aim: Human papillomaviruses (HPV) have frequently been detected in colorectal cancer tumor samples, and may play a role in the pathogenesis of colorectal cancer. This study was designed to investigate the presence of DNA and RNA for the high-risk HPV genotypes 16 and 18 in samples of colorectal cancer tumors and adjacent normal tissues. We also investigated the expression of proapoptotic genes in HPV-positive colorectal tumors compared to normal tissue samples. Methods: Samples of tumoral and adjacent normal tissues were fresh-frozen, and HPV DNA was identified by nested and semiquantitative PCR. Real time PCR was used to quantitatively compare the expression of HPV-18 E6 and nine proapoptotic genes in HPV-positive tumors and samples of adjacent normal tissue. Results: HPV-16 DNA was found in 10.5 of the tumor samples, and HPV-18 DNA was found in 23.6 of the samples. Real time PCR results showed lower expression of the E6 gene in HPV-positive tumors than in adjacent normal tissue. The expression of two proapoptotic genes, FAS and DR5, was significantly lower in tumor samples than in adjacent normal tissues. Conclusions: HPV infection, especially HPV-18, may play a role in colorectal cancer tumorigenesis by downregulating death receptor genes and interfering with the extrinsic pathway of apoptosis. (C) 2015 Elsevier GmbH. All rights reserved.

Item Type: Article
Keywords: Colorectal cancer Human papillomavirus Apoptosis polymerase-chain-reaction mediated apoptosis colon-cancer dna oncoproteins association virus adenocarcinoma prevalence carcinomas Pathology
Divisions:
Page Range: pp. 444-448
Journal or Publication Title: Pathology Research and Practice
Journal Index: ISI
Volume: 211
Number: 6
Identification Number: https://doi.org/10.1016/j.prp.2015.02.001
ISSN: 0344-0338
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5582

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