Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Recombinant human lipocalin 2 acts as an antibacterial agent to prevent platelet contamination

(2014) Recombinant human lipocalin 2 acts as an antibacterial agent to prevent platelet contamination. Hematology. pp. 487-492. ISSN 1024-5332

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Abstract

Background: Bacterial contamination of platelet products is the major infectious risk in blood transfusion medicine, which can result in life-threatening sepsis in recipient. Lipocalin 2 (Lcn2) is an iron-sequestering protein in the antibacterial innate immune response, which inhibit bacterial growth. This study was aimed to evaluate the antibacterial property of Lcn2 in preventing bacterial contamination of platelets. Methods: Recombinant Lcn2 was expressed in a eukaryotic expression system and following purification and characterization of the recombinant Lcn2, its minimum inhibitory concentration was determined. Then, platelet concentrates were inoculated with various concentrations of Staphylococcus epidermidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Enterococcus faecalis, and the antibacterial effects of Lcn2 was evaluated at 20-24 degrees C. Results: Results revealed that Lcn2 effectively inhibited the growth of 1.5 x 10(4) CFU/ml S. epidermidis, P. aeruginosa, K. pneumoniae, E. coli, and E. faecalis at 40 ng/ml. At this concentration, Lcn2 also inhibited the growth of 1.5 x 10(3) CFU/ml Staphylococcus aureus and Proteus mirabilis. Conclusion: Recombinant Lcn2 inhibited growth of a variety of platelet-contaminating bacteria. Therefore, supplementation of platelet concentrates with Lcn2 may reduce bacterial contamination.

Item Type: Article
Keywords: Platelet Bacterial infections Lcn2/NGAL Recombinant gelatinase-associated lipocalin early urinary biomarker infection Hematology
Divisions:
Page Range: pp. 487-492
Journal or Publication Title: Hematology
Journal Index: ISI
Volume: 19
Number: 8
Identification Number: https://doi.org/10.1179/1607845414y.0000000155
ISSN: 1024-5332
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5645

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