Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Modulation of human miR-17-3p expression by methyl 3-O-methyl gallate as explanation of its in vivo protective activities

(2014) Modulation of human miR-17-3p expression by methyl 3-O-methyl gallate as explanation of its in vivo protective activities. Molecular Nutrition & Food Research. pp. 1776-1784. ISSN 1613-4125

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Abstract

Scope: Methyl-3-O-methyl gallate (M3OMG) possesses in vivo antioxidant activity due to the partial restoration of the antioxidant enzymes, whose expression is altered in oxidative stress. Literature data suggest that miR-17-3p is a microRNA involved in the regulation of cellular redox status, interfering with transcription of the mRNAs responsible for the synthesis of antioxidant enzymes. To obtain deeper insight into the potential mechanism of action of M3OMG, the aim of this study was to investigate its effect on the expression levels of miR-17-3p in human cells. Methods and results: Peripheral blood mononuclear cells and EVC-304 cells were treated with increasing subtoxic concentrations of M3OMG. The expression levels of miR-17-3p, extracted from cells and exosomes, were determined by quantitative real-time PCR. M3OMG induced a decrease in the miR-17-3p levels, and an increase in the levels of mRNA coding for the antioxidant enzymes, when compared to the control samples. Differently, in exosomes the expression levels of miR-17-3p were depended on the compound, its concentration, and the type of cell. Conclusion: These results suggest a potential mechanism of action of M3OMG that, inducing the reduction of the levels of miR-17-3p and the increase of mRNA coding for antioxidant enzymes, allows to these latter to perform their protective effects.

Item Type: Article
Keywords: Antioxidant enzymes Human cells Methyl 3-O-methyl gallate MicroRNA miR-17-3p expression level induced oxidative stress peltiphyllum-peltatum microrna expression extracellular vesicles gene-expression messenger-rnas gallic acid antioxidant polyphenols identification Food Science & Technology
Divisions:
Page Range: pp. 1776-1784
Journal or Publication Title: Molecular Nutrition & Food Research
Journal Index: ISI
Volume: 58
Number: 9
Identification Number: https://doi.org/10.1002/mnfr.201400007
ISSN: 1613-4125
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5733

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