Abstract

Background and purpose: Properties of biocompatibility and biodegradation are important factors in choosing a polymer to prepare nano-fiber for medical and biological purposes. PLGA high attention in tissue engineering for the above properties, the aim of present study is the evaluation of rat bone marrow stromal cells cultured on PLGA nanofibers. Material and methods: In this study, the PLGA nanofibers were prepared using electrospinning technique in hexa fluoro propanol solvent. To study the nanofiber properties scanning electron microscopy (SEM) and invert microscope were used. After bone marrow stromal cells culture (BMSCs) and achieving passage two, the cells were grown in two groups of PLGA nanofibers and without nanofibers. Life and death of cells were examined in second, fourth, and sixth days by Acridine Orange. Also, the morphology of cells was investigated via light microscopy. Results: The results showed no significant reduction in cell growth in PLGA nanofibers compared with that of the control group. Conclusion: According to the results obtained from BMSCc culture, PLGA nanofibers could be used as biodegradable and biocompatible scaffold alongside bone marrow cells in tissue engineering. © 2014 Mazandaran University of Medical Sciences. All Rights Reserved.