(2013) In vitro evaluation of biomimetic nanocomposite scaffold using endometrial stem cell derived osteoblast-like cells. Tissue & Cell. pp. 328-337. ISSN 0040-8166
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Abstract
Current study encourages the differentiation of human endometrial stem cells into osteoblast-like cells using osteogenic media for potential bone tissue engineering purposes. A biomimetic nanocomposite scaffolds based on GEL/calcium phosphate were fabricated and behavior of differentiated osteoblast cells was evaluated after seeding on this scaffold. Prepared scaffolds were assessed in terms of attachment, alkaline phosphatase activity, gene expression and proliferation of osteoblast cells. The matrix mineralization was approved by alizarin red and the treated cultures with osteogenic media and BMP2 were positive for osteopontin and osteocalcin antibodies. RT-PCR confirmed presence of osteopontin, osteonectin and alkaline phosphatase mRNA after differentiation in EnSCs-derived osteoblast-like cells. Also, it has been shown that the biomimetic nanocomposites possess appropriate chemical and physical properties to support the attachment and proliferation of differentiated osteoblast cells. (C) 2013 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Keywords: | Endometrial stem cells Osteoblast cells Biomimetic nanocomposite Bone tissue engineeringa amorphous calcium-phosphate bone morphogenetic proteins marrow stromal cells composite scaffolds chitosan membrane apatite formation diffusion system hydroxyapatite regeneration adult Anatomy & Morphology Cell Biology |
| Divisions: | |
| Page Range: | pp. 328-337 |
| Journal or Publication Title: | Tissue & Cell |
| Journal Index: | ISI |
| Volume: | 45 |
| Number: | 5 |
| Identification Number: | https://doi.org/10.1016/j.tice.2013.05.002 |
| ISSN: | 0040-8166 |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.bmsu.ac.ir/id/eprint/5962 |
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