Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Using Taguchi Statistical Method for the Determination of Suitable Conditions for Cloning and Over Expression of N-terminal region of ipad in Escherichia coli

(2013) Using Taguchi Statistical Method for the Determination of Suitable Conditions for Cloning and Over Expression of N-terminal region of ipad in Escherichia coli. Journal of Pure and Applied Microbiology. pp. 1851-1860. ISSN 0973-7510

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Abstract

IpaD is one of the most important virulence factors in Shigella dysentriae. Recent studies showed that N-terminal region of IpaD has a major role in bacterial invasion and it could be considered as a suitable candidate for vaccine development against S. dysentriae. In this study, the gene was cloned into Ecoli in order to have sufficient amount of protein and subsequently the recombinant protein was purified. So as to optimize protein expression, Taguchi statistical method was used. After optimization of the method, Protein expression was increased by 2.2 fold compared to control. This study showed that the K2HPO4/KH2PO4 ratio and OD600nm of Induction are the main factors in the expression of the recombinant IpaD by E.coli BI21 DE3, while other variables have slight effects on the expression procedure.

Item Type: Article
Keywords: IpaD Optimization S. dysentriae Taguchi Statistical Method shigella-flexneri optimization binding needle tip recruitment protein Biotechnology & Applied Microbiology Microbiology
Divisions:
Page Range: pp. 1851-1860
Journal or Publication Title: Journal of Pure and Applied Microbiology
Journal Index: ISI
Volume: 7
Number: 3
ISSN: 0973-7510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/5972

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