Abstract

Resistant strains of Uropathogenic Escherichia coli have been considered as the most prevalent cause of UTIs. Recently, the incidence of extended spectrum beta lactamases producing E. coli has been increased and causes severe illnesses. Resistance to β-lactam antimicrobial agents in E. coli is primarily mediated by β-lactamases. The classical TEM-1 enzyme is the predominant plasmid-mediated β-lactamases of gram-negative rods. This study aimed to detect E. coli in urine samples from patients at the valiasr hospital, Rasht, Iran to study the antibiotic susceptibility pattern of bacterial isolates as well as analyze the distribution of ESBL strains and TEM-1 enzyme. Seven hundred thirty one urine samples of hospitalized patients and Outpatient of valiasr hospital were collected and immediately cultured. Presence of ESBL strains and TEM-1 enzyme have been diagnosed using the PCR method. Susceptibility of E. coli isolates against 9 commonly used antibiotics has been studied usimg the disk diffusion method. Of 731 urine samples, 149 samples (20.38) were positive for E. coli. Women had the higher distribution of E. coli than men (P<0.05). We found that 30-40 years old patients had the highest incidence of E. coli. E. coli strains had the highest levels of resistance to amoxicillin (97.31), cephalexin (80.53), tetracycline (75.16) and co amoxiclav (69.12). Of 149 E. coli isolates, 58 samples were ESBL (38.92) and the incidence of TEM-1 enzyme was 21.47. Special health care should be performed on management of Urinary tract infections in public place. We suggested use of gentamicin and ciprofloxacin antibiotics in order to treatment of UTIs in patients. High presence of ESBL strains suggested the need to orient much attention over antibiotics.