Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Heterologous Expression of Staphylococcal Enterotoxin E Gene

(2013) Heterologous Expression of Staphylococcal Enterotoxin E Gene. Journal of Pure and Applied Microbiology. pp. 957-963. ISSN 0973-7510

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Abstract

One of the main virulence factors of S.aureus is a superantigene, named enterotoxin type E. The entE gene (693 bp) was isolated from the native strains of S. aureus. It was amplified with PCR and sequenced. The entE gene was cloned into a pET-28a expression vector. Then, the recombinant plasmids were electroporatively transformed into E. coli Rosetta BL-21(DE3) strain and was expressed. The recombinant entE was purified and confirmatory tests were performed. The results showed the specific pattern and its similarity with the reference entE gene. The recombinant protein (similar to 30Kda) was confirmed by western blotting. RFLP pattern, sequencing and western blotting of the gene product confirmed the entE gene. Therefore, the method of cloning and expression of entE gene was set up and paving the way for the production of specific antibody and achievement of rapid diagnostic method for SEE.

Item Type: Article
Keywords: Staphylococcus aureus Recombinant protein Enterotoxin E Cloning Expression physicochemical properties nucleotide-sequence aureus toxin identification purification Biotechnology & Applied Microbiology Microbiology
Divisions:
Page Range: pp. 957-963
Journal or Publication Title: Journal of Pure and Applied Microbiology
Journal Index: ISI
Volume: 7
Number: 2
ISSN: 0973-7510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/6024

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