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Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Design of a multiplex PCR method for detection of toxigenic- pathogenic in Vibrio cholerae

(2013) Design of a multiplex PCR method for detection of toxigenic- pathogenic in Vibrio cholerae. Asian Pacific Journal of Tropical Medicine. pp. 115-118. ISSN 1995-7645

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Abstract

Objective: To study virulence and regulatory genes (hlyA, ctxB, up in clinical strains of Vibrio cholerae (V. cholerae), simultaneously. Methods: Three important genes, lcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method. Results: According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7 (72 sample), 90.8 (69 sample), and 92.1 (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI. Conclusions: Result show that this method could be reliable to detect toxigenic pathogenic strains of V. cholerae in Iran.

Item Type: Article
Keywords: Vibrio cholerae Multiplex PCR Rapid detection virulence genes molecular characterization outbreak strains non-o139 strains rapid detection o1 biotype assay Public, Environmental & Occupational Health Tropical Medicine
Divisions:
Page Range: pp. 115-118
Journal or Publication Title: Asian Pacific Journal of Tropical Medicine
Journal Index: ISI
Volume: 6
Number: 2
Identification Number: https://doi.org/10.1016/s1995-7645(13)60005-x
ISSN: 1995-7645
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/6110

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