Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Microbial Susceptibility, Virulence Factors, and Plasmid Profiles of Uropathogenic Escherichia coli Strains Isolated from Children in Jahrom, Iran

(2012) Microbial Susceptibility, Virulence Factors, and Plasmid Profiles of Uropathogenic Escherichia coli Strains Isolated from Children in Jahrom, Iran. Archives of Iranian Medicine. pp. 312-316. ISSN 1029-2977

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Abstract

Background: Urinary tract infections (UTIs), including cystitis and pyelonephritis, are the most common infectious diseases in childhood. Escherichia coli (E. coli) accounts for as much as 90 of the community-acquired and 50 of nosocomial UTIs. Therefore, identification of E. coli strains is important for both clinical and epidemiological implications. Understanding antibiotic resistance patterns and molecular characterization of plasmids and other genetic elements is also epidemiologically useful. Methods: To characterize uropathogenic strains of E. coli, we studied 96 E. coli strains recovered from urine samples of children aged 1 month to 14 years with community-acquired UTIs in Jahrom, Iran. We assessed virulence factors (VFs), drug sensitivities, and plasmid profiles. Results: Drug sensitivities of the isolates were: 19.8 (ampicillin), 24 (trimethoprim-sulfamethoxazole), 29.2 ( tetracycline), 75.5 (nalidixic acid), 80.4 (cefixime), 84.6 (gentamicin), 91.4 (ciprofloxacin), 96.8 (nitrofurantoin), 96.8 (amikacin) and 100 (imipenem). Totally, 76 isolates harbored plasmids with an average of 5.5 plasmids (range: 1 - 10) in each strain. Plasmid profiling distinguished 22 different E. coli genotypes in all isolates that ranged in similarity from 50 to 100. PCR showed that the prevalence of virulence genes ranged from 15.62 for hly to 30.2 for pap. Conclusion: These data mandate local monitoring of drug resistance and its consideration in empirical therapy of E. coli infections. Plasmid analysis of representative E. coli isolates also demonstrates the presence of a wide range of plasmid sizes, with no consistent relationship between plasmid profiles and resistance phenotypes. Plasmid profiles distinguished more strains than did the antimicrobial susceptibility pattern.

Item Type: Article
Keywords: E. coli plasmid UTI virulence genes urinary-tract-infection polymerase-chain-reaction pathogenicity islands multidrug-resistance united-states prevalence integrons General & Internal Medicine
Divisions:
Page Range: pp. 312-316
Journal or Publication Title: Archives of Iranian Medicine
Journal Index: ISI
Volume: 15
Number: 5
ISSN: 1029-2977
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/6322

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