(2011) OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response. Cancer Letters. pp. 170-175. ISSN 0304-3835
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OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response.pdf Download (759kB) |
Abstract
A novel variant of OCT4, OCT4B1, is highly expressed in pluripotent cells. OCT4B1 is also upregulated in various tumors and cell lines. Here, we have constructed a HA-tagged OCT4B1 construct, which could be translated into an N-terminally tagged protein, detectable by HA antibody. Western blotting revealed that OCT4B1 can act as a precursor for OCT4B, and it can also produce a truncated protein with a molecular size corresponding to that of predicted OCT4B1. Furthermore, our data demonstrated a cytoplasmic distribution for both isoforms. Interestingly, the expression ratio of OCT4B1/OCT4B transcripts and proteins was significantly elevated under the heat-stress condition. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Keywords: | OCT4B1 Cancer stem cell OCT4B Bladder cancer Heat stress embryonic stem-cells transcription factor oct4 self-renewal mammalian embryo pou-domain expression isoforms marker pluripotent biology Oncology |
| Divisions: | |
| Page Range: | pp. 170-175 |
| Journal or Publication Title: | Cancer Letters |
| Journal Index: | ISI |
| Volume: | 309 |
| Number: | 2 |
| Identification Number: | https://doi.org/10.1016/j.canlet.2011.05.027 |
| ISSN: | 0304-3835 |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.bmsu.ac.ir/id/eprint/6476 |
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