Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response

(2011) OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response. Cancer Letters. pp. 170-175. ISSN 0304-3835

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Abstract

A novel variant of OCT4, OCT4B1, is highly expressed in pluripotent cells. OCT4B1 is also upregulated in various tumors and cell lines. Here, we have constructed a HA-tagged OCT4B1 construct, which could be translated into an N-terminally tagged protein, detectable by HA antibody. Western blotting revealed that OCT4B1 can act as a precursor for OCT4B, and it can also produce a truncated protein with a molecular size corresponding to that of predicted OCT4B1. Furthermore, our data demonstrated a cytoplasmic distribution for both isoforms. Interestingly, the expression ratio of OCT4B1/OCT4B transcripts and proteins was significantly elevated under the heat-stress condition. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

Item Type: Article
Keywords: OCT4B1 Cancer stem cell OCT4B Bladder cancer Heat stress embryonic stem-cells transcription factor oct4 self-renewal mammalian embryo pou-domain expression isoforms marker pluripotent biology Oncology
Divisions:
Page Range: pp. 170-175
Journal or Publication Title: Cancer Letters
Journal Index: ISI
Volume: 309
Number: 2
Identification Number: https://doi.org/10.1016/j.canlet.2011.05.027
ISSN: 0304-3835
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/6476

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