(2011) p75NTR suppression in rat bone marrow stromal stem cells significantly reduced their rate of apoptosis during neural differentiation. Neuroscience Letters. pp. 15-19. ISSN 0304-3940
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Abstract
Most of the transplanted cells within central nervous system (CNS) undergo extensive cell death. Preventing the death of stem cell-derived neuron-like cells within adult CNS would enhance the efficiency of transplantation in clinics. We have employed an interfering RNA (RNAi) approach to elevate the survival rate of neurally differentiated bone marrow stromal stem cells (BMSCs), by means of suppressing p75NTR expression. Our data revealed that stably overexpressing a specific shRNA against p75NTR transcript could effectively reduce the expression of endogenous p75NTR in neurally differentiated BMSCs. As p75NTR can induce neuronal death in target cells, its suppression is followed by a significant reduction of apoptosis in neural-like cells derived from BMSCs. Thus, our data provides a method to increase the survival of stem cells being employed in transplantation within CNS and hence increase the success rate of cell-based therapies in damaged area of brain and spinal cord. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Keywords: | Apoptosis p75NTR BMSC RNAi Neuronal differentiation Cell therapy flow-cytometric detection annexin-v phosphatidylserine expression neuronal apoptosis spinal-cord in-vitro death induction proneurotrophins neurotrophins Neurosciences & Neurology |
| Divisions: | |
| Page Range: | pp. 15-19 |
| Journal or Publication Title: | Neuroscience Letters |
| Journal Index: | ISI |
| Volume: | 498 |
| Number: | 1 |
| Identification Number: | https://doi.org/10.1016/j.neulet.2011.04.050 |
| ISSN: | 0304-3940 |
| Depositing User: | مهندس مهدی شریفی |
| URI: | http://eprints.bmsu.ac.ir/id/eprint/6539 |
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