Repository of Research and Investigative Information

Repository of Research and Investigative Information

Baqiyatallah University of Medical Sciences

Inhibitory effects of arsenic trioxide and thalidomide on angiogenesis and vascular endothelial growth factor expression in leukemia cells

(2018) Inhibitory effects of arsenic trioxide and thalidomide on angiogenesis and vascular endothelial growth factor expression in leukemia cells. Asian Pacific Journal of Cancer Prevention. pp. 1127-1134. ISSN 15137368 (ISSN)

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Abstract

Acute myeloid leukemia (AML) is a blood disorder characterized by uncontrolled proliferation of myeloid progenitors and decrease in the apoptosis rate. The vascular endothelial growth factor (VEGF) promotes blood vessel regeneration which might play important roles in development and progression of neoplasia. Our previous studies focused on cytotoxicity and anticancer effects of arsenic trioxide (ATO) and thalidomide (THAL) as an anti-VEGF compound in the AML cell model. ATO also affects regulatory genes involved in cell proliferation and apoptosis. The aim of present study was to examine the effects of ATO and THAL alone and in combination on U937 and KG-1 cells, with attention to mRNA expression for VEGF isoforms. Growth inhibitory effects was assessed by MTT assay and apoptosis induction was determined by Annexin/PI staining. mRNA expression levels were evaluated by real-time PCR. Our data indicated that ATO (1.618μM and 1μM in KG-1 and U937 cell lines respectively), THAL (80μM and 60μM) and their combination inhibited proliferation and induced apoptosis in our cell lines. mRNA expression of VEGF (A, B) decreased while C and D isoforms did not show any significant changes. Taken together, according to the obtained results, the VEGF autocrine loop could be a target as a therapeutic strategy for cases of AML. © 2017, Asian Pacific Organization for Cancer Prevention.

Item Type: Article
Keywords: Acute myeloid leukemia Arsenic trioxide Thalidomide Vascular Endothelial Growth Factor (VEGF) antineoplastic agent organoarsenic derivative oxide vasculotropin apoptosis cell proliferation cell survival drug effect human metabolism neovascularization (pathology) tumor cell line U-937 cell line Antineoplastic Agents Arsenicals Cell Line, Tumor Humans Leukemia, Myeloid, Acute Neovascularization, Pathologic Oxides U937 Cells Vascular Endothelial Growth Factors
Divisions:
Page Range: pp. 1127-1134
Journal or Publication Title: Asian Pacific Journal of Cancer Prevention
Journal Index: Scopus
Volume: 19
Number: 4
Identification Number: https://doi.org/10.22034/APJCP.2018.19.4.1127
ISSN: 15137368 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.bmsu.ac.ir/id/eprint/658

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